Journal: International Journal of Molecular Sciences

Article Title: Integrated Metabolomics and Transcriptomics Analysis of Anacardic Acid Inhibition of Breast Cancer Cell Viability

doi: 10.3390/ijms25137044

Figure Lengend Snippet: Anacardic acid (AnAc) inhibits the viability of ER+ and TNBC cells. Each cell line was grown in 5% DCC-FBS-phenol red-free IMEM for 48 h prior to 48 h treatment with EtOH (ethanol, the vehicle control) or the indicated concentration of AnAc for 48 h prior to MTT assay. Values are the mean + SEM of 4 replicates within one experiment. IC 50 values from repeated experiments are in . ( A – E ) are the viability plots for MCF-7, MDA-MB-231, MDA-MB-468, HCC1806, and BT-20 BC cell lines, respectively.

Article Snippet: For metabolomics, cells were grown to 75–80% confluence in phenol red-free IMEM (ThermoFisher, Waltham, MA, USA) medium containing 5% dextran-coated charcoal (DCC)-stripped FBS (hormone-depleted medium) in a T-75 flask for 48 h prior to treatment with the ~IC 50 for AnAc 24:1n5 (hereafter AnAc) for 24 h ( ).

Techniques: Control, Concentration Assay, MTT Assay

Journal: International Journal of Molecular Sciences

Article Title: Integrated Metabolomics and Transcriptomics Analysis of Anacardic Acid Inhibition of Breast Cancer Cell Viability

doi: 10.3390/ijms25137044

Figure Lengend Snippet: Principal Component Analysis (PCA) and Orthogonal Partial Least Squares - Discriminant Analysis (OPLS-DA) of metabolites from the control (EtOH) ( A , C , E ) and AnAc-treated ( B , D , F ) cell lines. Each point is a separate replicate sample from the indicated cell lines. Each cell line was grown in 5% DCC-FBS-phenol red-free IMEM for 48 h prior to 24 h treatment with EtOH (vehicle control) or AnAc for 24 h. PCA scores of plots of all metabolites: 3D score plots ( A , B ) and 2D score plots ( C , D ) of the same samples reveals a distinct metabolic signature of MCF-7 and MDA-MB-231 cells from the other three TNBC cell lines: BT-20, HCC1806 and MDA-MB-468. OPLSA-DA score plots identify distinct metabolic signatures of each cell line with BT-20 and HCC1806 showing lower T scores compared to MCF-7, MDA-MB-231 and MDA-MB-468 cells.

Article Snippet: For metabolomics, cells were grown to 75–80% confluence in phenol red-free IMEM (ThermoFisher, Waltham, MA, USA) medium containing 5% dextran-coated charcoal (DCC)-stripped FBS (hormone-depleted medium) in a T-75 flask for 48 h prior to treatment with the ~IC 50 for AnAc 24:1n5 (hereafter AnAc) for 24 h ( ).

Techniques: Control

Journal: International Journal of Molecular Sciences

Article Title: Integrated Metabolomics and Transcriptomics Analysis of Anacardic Acid Inhibition of Breast Cancer Cell Viability

doi: 10.3390/ijms25137044

Figure Lengend Snippet: Metabolite differences between cell lines and in response to AnAc treatment. Heatmaps of unsupervised hierarchical cluster analysis of the top 25 metabolite concentrations in the metabolomics data from the control (EtOH) ( A ) and AnAc-treated ( B ) cell lines. The rows show different metabolites in EtOH ( A ) and AnAc-treated cells ( B ) with the columns indicating the individual cell lines. ( C – G ) OPLS-DA of metabolites from the control (EtOH) and AnAc-treated cell lines. ( C ): MCF-7, ( D ): BT-20, ( E ): HCC-1806, ( F ): MDA-MB-231, and ( G ): MDA-MB-468 BC cells. Each point is a separate replicate sample from the indicated cell lines. Each cell line was grown in 5% DCC-FBS-phenol red-free IMEM for 48 h prior to 24 h treatment with EtOH or AnAc. OPLSA-DA score plots identify distinct metabolic signatures with AnAc treatment of each cell line.

Article Snippet: For metabolomics, cells were grown to 75–80% confluence in phenol red-free IMEM (ThermoFisher, Waltham, MA, USA) medium containing 5% dextran-coated charcoal (DCC)-stripped FBS (hormone-depleted medium) in a T-75 flask for 48 h prior to treatment with the ~IC 50 for AnAc 24:1n5 (hereafter AnAc) for 24 h ( ).

Techniques: Control